--- Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au>
wrote:

 can not find a protocol or a kit that would
> allow me to achieve this with two antibodies raised
> in the same species.

I am also a student-my opinion is that, if you use
primary antibodies raised in same species how will you
know what the secondary antibody is recognising.
The alternative is to use  primary antibodies that are
tagged to different labels. the specificity of such
detection will be low.
Sunil Thomas K 

> Histonetters,
> 
> I am a student in the department of Zoology at the
> University of Melbourne, Australia.  I have two
> antibodies raised in the same species however I need
> to co-localize their  expression in the one tissue
> section.  I have protocols for immuno double
> staining of antibodies raised in different species,
> but I can not find a protocol or a kit that would
> allow me to achieve this with two antibodies raised
> in the same species.  I am currently using
> immunofluorescence and would like to remain with
> this visualization.  I would be very grateful for
> any help.
> 
> Thanks 
> 
> 
> Doug  
> 
> BRUCE ABALOZ
> HISTOLOGIST		      
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