We use DAKO poly c-kit at1:50 on the Ventana Nexes for 32 minutes.
epitope retrieval is citrate buffer in the pressure cooker. We will switch
this ab to the DAKO autostainer using envision +
Rena Fail, AS,HT(ASCP)
SS/IHC Medical University of SC
At 10:03 AM 7/27/01 -0500, you wrote:
> Hi there,
> Could anyone please help out with a protocol for running CD117? We
>have been validating the Santa Cruz polyclonal (goat) and the Dako
>polyclonal (rabbit) and are having some difficulties getting consistent
> A test protocol would be very helpful, including method of antigen
>retrieval, dilution factors, instrumentation, etc.
>Thank you very much!!!!!!
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