Dan,
      We use DAKO poly c-kit  at1:50 on the Ventana Nexes  for 32 minutes. 
epitope retrieval is citrate buffer in the pressure cooker.  We will switch 
this ab to the DAKO autostainer using envision +

Rena Fail, AS,HT(ASCP)
SS/IHC Medical University of SC
Charleston SC

At 10:03 AM 7/27/01 -0500, you wrote:
>  Hi there,
>         Could anyone please help out with a protocol for running CD117?  We
>have been validating the Santa Cruz polyclonal (goat) and the Dako
>polyclonal (rabbit) and are having some difficulties getting consistent
>results.
>         A test protocol would be very helpful, including method of antigen
>retrieval, dilution factors, instrumentation, etc.
>
>Thank you very much!!!!!!