Re: Bouins and alcohol

From:Geoff McAuliffe

"Gaskill, Betty A" wrote:

>  Does anyone know a minimum amount of time bouins fixed tissue should stay
> in 70% alcohol before being processed?  A few hours?
> Any info would be appreciated.
> Betty

I was taught to use 4 changes of 50% ethanol, 60 to 90 minutes each, to remove
the picric acid from the tissue (relatively small pieces of rodent tissues,
longer time might be needed for larger tissues?). Inadequate removal of
picrates before embedding leads to long-term damage to the tissue. This can be
a factor if sections are needed later. The artifact section of the third
edition of the AFIP manual shows the damage.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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