Elizabeth,
It is 5ml distilled water, 2 drops buffer, 4 drops DAB, 2 drops H2O2 then 2
drops of the nickel.
Good Luck!
Mary


> ----------
> From: 	Elizaha@aol.com
> Sent: 	Monday, July 23, 2001 9:19 AM
> To: 	histonet@pathology.swmed.edu
> Subject: 	vector DAB kit
>
>    Hi,
>
> I have lost the protocol for mixing the Vector kit for Dab. I was
> wondering
> if someone could refresh my memory (such as it is).
>
> I think it was 5 ml H20, add three drops buffer, 1 drop DAB, then 1 drop
> H2O2.
> And I can't remember at all how much of the nickel.
>
>