RE: paraffin section compression
|From:||"Brennan, Liam" <Liam.Brennan@bll.n-i.nhs.uk>|
Carla- try floating your sections onto a cold ethanol bath, this should
expand the section and get rid of the wrinkles.
> Hi everyone,
> Our lab was sent blocks of fish tissue (liver, spleen and heart) embedded
> in Paraplast X-tra (aka Tissue-Path). I am trying to cut sections at 4
> microns. They are compressed when cut and paraffin around the tissue
> doesn't want to expand on the water bath causing lots of wrinkles(on
> tissue, not me yet). Here's what I've tried so far:
> - after sectioning, cutting around the tissue radially and trying to
> out with brush (didn't help much)
> - adjusting the knife angle (cuts best at 3 deg.)
> - raising water bath temp. (49C )
> - soaking blocks in ice bath from 10 min. to half day
> - changing disposable knives constantly
> - slowing my cutting speed (sectioning done manually)
> Slides will be given to participants at a workshop, so I'd like to make
> them as perfect as possible.
> Please help- I am running out of time, tissue and ideas.
> Thank you,
> Carla (Aiwohi) Conway
> Western Fisheries Research Center
> Seattle, WA
> P.S. Do others have trouble cutting Paraplast-embedded tissues (or is it
> just me)?
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