Osteoid seams can be seen in H & E stain but Masson's Trichrome, Safranin
O/Fast Green and other Trichrome staining techniques can highlight the
osteoid areas in bone. Depends on what colors the observer wishes to view.

Regarding muscle fiber types, PTAH is beautiful for striated muscle.
My two cents, Donna Montague, M.S.
Research Associate
Physiology/Biophysics and Orthopaedic Surgery
University of Arkansas for Medical Sciences
(501) 603-1239


-----Original Message-----
From: Jill Songer [mailto:jtsonger@vt.edu]
Sent: Monday, July 30, 2001 3:01 PM
To: histonet@pathology.swmed.edu
Subject: osteoid stain


Hi fellow histonetters,

Actually, I have 2 questions:

1. What are you using to stain osteoids?

2. Do you know if there is a method to differentiate muscle fiber type for 
FFPE equine?

Thanks as always

**************************************************************************
Jill Songer HT (ASCP)
Supervisor, Anatomic Pathology
Virg