Garry,

In short, no. Sounds like one of those "that's the way we've always done it"
things. I have used the DAKO polyclonal and a 15' room temp digest with
their Proteinase K. No problem.

Brett

Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075
e-mail. brett_connolly@merck.com


-----Original Message-----
From: Garry Ashton [mailto:GAshton@picr.man.ac.uk]
Sent: Monday, July 16, 2001 11:00 AM
To: 'histonet'
Subject: Von Willebrand Factor staining


Dear all,
As some of you may be aware from previous e-mails I am currently
immunostaining FFPE sections to identify blood vessels.
Does anybody have a good reason why on a protocol I have been shown,  the
sections are treated with 0.2% proteinase (protease I think) for 8 mins at
37 degrees, immediately followed  after washing by the more conventional Ag
retrieval method using trypsin.
Any help and ad