RE: Tissue processing - small biopsies-Histogel

From:marjorie lehman

I have used Histogel for really small samples and it works like a dream. The
sections are better than those embedded in gelatin too. I do like the
upside-down syringe idea, but the Histogel does not become as firm as gelatin,
so it might get squished when you try to expel it.
Marge L.

-----Original Message-----
From:	Morken, Tim []
Sent:	Tuesday, July 31, 2001 7:48 AM
To:	'Histonet'
Cc:	''
Subject:	RE: Tissue processing - small biopsies


Wow! you pratically described a product called Histogel. In the US it is
available from Lab Storage Systems, amoung others.

See: and look under Histology Supplies in the search

Tim Morken
CDC, Atlanta

-----Original Message-----
From: Coaker, Terry []
Sent: Tuesday, July 31, 2001 6:07 AM
To: 'Histonet'
Subject: Tissue processing - small biopsies

What is the most secure, reliable, quick and cost effective way of
processing small biopsies in Tissue Tek II type cassettes?  

Metal, plastic and foam inserts, filter paper, tea bag paper, agar
orientation technique - all have pros and cons.

What is really neeeded, is an agar type substitute.  You place the tisssue
on to / into this stuff in the required orientation.  The tissue sticks to
it.  The stuff is resistant to solvents on a processor.  You embed it on its
flat surface.  No wrapping needed, orientation correct - sorted.  
Does anyone know the name of this compound ?


Terry Coaker

Chief Biomedical Scientist
Cellular Pathology
Royal Victoria Infirmary
Newcastle upon Tyne NE1 4LP
0191 282 4445


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