RE: Slide drying

From:David Taylor Manager <DTMan@kingmower.com.au>

Dear Charlene, after a paraffin section is mounted onto the slide we
drain vertically for 2-3 minutes, (don't let sections dry out
completely). Then the slide is blotted firmly between multiple layers of
damp whatman's No.1 filter paper using the flat part of a clenched fist,
(you can also use an art roller). When blotting apply firm even pressure
starting at the top of the slide. The slide is then placed on a flat 60C
hot-plate for about 5 minutes. Slides then get loaded into a staining
rack, blow-dried briefly with a hair-drier to re-melt the wax and then
placed into xylene, xylene, alc, etc etc.
We cut a wide variety of human and animal tissue and require very few
sections to be placed on coated slides.
It's important to remember these are only a few of the steps to produce
a good H+E. You need blocks that are well fixed, processed, embedded,
trimmed carefully with sharp blades at room temp, cooled on a cold
plate, the sharpest blades you can find, a good microtome that is well
maintained, etc. David.

David Taylor
Laboratory Manager
Drs King & Mower
Adelaide, Australia


-----Original Message-----
From: Henry, Charlene [mailto:Charlene.Henry@stjude.org]
Sent: Thursday, 19 July 2001 06:25
To: 'histonet@pathology.swmed.edu'
Subject: Slide drying


Thanks for all of the responses about slide drying ovens. Our
pathologist
ask that I also ask the following question. After cutting surgical
paraffin
slides, how long and at what temperature do you dry your slides? Also do
you
use an oven, microwave, hotplate or ect? Any

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