RE:LFB wash off
I do A LOT of brains here and a lot of LFB stains. I put my sections on
Snowcoat X-tra slides that I get from Surgipath and have never had a
section float off (imagine the sound of my knocking on wood here). I put DI
water in the water bath and dry the slides in an oven at 58 degrees.
I do the overnite version of the LFB and finish it off with PAS. Reference
is Preece, 3rd edition.
Andi
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
:...................................................................:
http://www.cba.ar
<< Previous Message | Next Message >>