Try using a charged slide - let air dry overnight before oven drying. This
has worked for me! Good luck. j 

> -----Original Message-----
> From:	Michelle Rederick [SMTP:MRederick@neo.rr.com]
> Sent:	Wednesday, July 25, 2001 9:16 PM
> To:	Histonet
> Subject:	LFB
> 
> Hello Histonetters!
> 
> I am having a problem with a Luxol Fast Blue Stain that I am doing on a
> section of brain for the HTL registry. I have run the stain several times
> and every time the sections have washed off of the slides. I have tried
> using gelatin, poly-l-lysine, and even Elmer's glue in the water bath to
> remedy this problem.
> 
> The section stayed on once when I used a double coating of Poly, but then
> the stain came out very uneven and there was a deep blue staining where
> the
> poly had been. Does anyone have any suggestions? The deadline for the
> registry is looming! Thanks in advance.
> 
> Michelle Rederick  HT(ASCP)
> Sharon Regional Health System
> 74