Best DAB I have ever used is premade from Research Genetics called Stable
Dab cat. #750118, you store it in the freezer but it does not freeze (has
glycerine or something to keep it liquid), you just pour it out and use it,
it never percipitates and is cheap also.
1-800-533-4363  developed by David Bragati (remember him?)

		-----Original Message-----
		From:	Georger, Mary
[mailto:Mary_Georger@URMC.Rochester.edu]
		Sent:	Monday, July 30, 2001 2:02 PM
		To:	'Histonet'
		Subject:	Immuno Reagents

		Good Afternoon All,
		I have a question for those of you out there doing Immunos.
I am having a
		problem with the Vector DAB kit that I just bought, when you
add the DAB
		drops to the buffered double distilled water it immediately
turns dark
		brown. This has not happened with any previous kits. To make
matters worse I
		am in the middle of a study and hate to risk any study
slides with this
		chromagen. I called technical support for Vector and was
told I could fed-ex
		back the kit and they would do another QC on it, but in the
interim I would
		have to buy an additional kit to finish the work I am in the
middle of.
		 Now I have been off the bench for 2 years, but it seemed to
me that
		previously when there was a question with a reagent the
supplier sent out a
		replacement first so that you were not left high and dry.
This has still
		been my experience with DAKO, they have been great! 
		My question- Is this type of non-service the hallmark for
companies now? 
		I have learned a valuable lessen about who I will buy from
in the future.
		Mary Georger
		U