RE: IHC Double staining

From:Robert Geske <RGeske@lexgen.com>

tag each antibody with a different fluorochrome.  molecular probes sells
excellent kits for this purpose.


http://www.molecularprobes.com/

rob

-----Original Message-----
From: Bruce Abaloz
To: histoNet@Pathology.swmed.edu
Sent: 7/18/01 7:33 PM
Subject: IHC Double staining

Histonetters,

I am a student in the department of Zoology at the University of
Melbourne, Australia.  I have two antibodies raised in the same species
however I need to co-localize their  expression in the one tissue
section.  I have protocols for immuno double staining of antibodies
raised in different species, but I can not find a protocol or a kit that
would allow me to achieve this with two antibodies raised in the same
species.  I am currently using immunofluorescence and would like to
remain with this visualization.  I would be very grateful for any help.

Thanks 


Doug  

BRUCE ABALOZ
HISTOLOGIST		      
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The UNIVERSITY of MELBOURNE        *  FAX:   +61 3 83447909 
Parkville, Victoria.3010           *  EMAIL:
b.abaloz@zoology.unimelb.edu.au

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