RE: Daily Digest
| From: | "ryandes@esatclear.ie" |
Hello S. Clarke,
I also had trouble with fading of pens I got from TBS and had to revert
back to my old reliable pencil. Some cassettes are better than others
(smoother) for pencil. A 2B is best and you won't have to put it in the
budget.
Annette Ryan
Medical Laboratory Technician,
Our Lady of Lourdes Hospital,
Drogheda,
Co Louth,
Ireland
At 12:28 PM 7/30/01 -0400, SClark@mhs-net.com wrote:
>I would like to know if anyone is having trouble with the writing coming off
>of their cassettes. We are using Securline Marker 11/ Superfrost, from
>Baxter. Somedays we open the processors and the writing is off on part off
>the cassettes. One day we opened up the processor and about 12 cassettes had
>no writing. We were denied a cassette labeler in this years budget. I was
>wondering if anyone could share any tips on these pens are any other
>suggestions.
>
>S. Clark (sclark@mhs-net.com)
>
> -----Original Message-----
> From: HistoNet Server
>[mailto:histonet@pathology.swmed.edu]
> Sent: Sunday, July 29, 2001 1:09 AM
> To: HistoNet Server
> Subject: Daily Digest
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 00:30:43 -0500
> From: "Richard N Powell"
> Subject: RE: Top Atomic Pathologists and OHS issues in US
>labs
>
> Have to agree!
>
> Most US citizens don't seem to know that the big round thing
>we live on has
> other countries - its amazing to see the number of US based
>web sites that
> don't understand there is a big world out there with 60 to
>70% more web
> users than there are in te US! Having said that, I have had
>the pleasure of
> meeting several great American Pathologists - funny though
>they don't seem
> to be any different than our local ones..........
>
> As to the thread on OHS issues surrounding flammables in
>histo laboratories.
> Does the US have any uniform OHS Laws or are they all local
>State laws?
> Surely your accreditation bodies also have a brief to look
>at safety in
> laboratories?
>
> I guess that also begs the question of how many US labs are
>actually
> accredited, have quality systems, ISO 9000 accreditation or
>ISO/IEC 17025
> accreditation?
>
> My impression from these pages is that there is a huge
>variation in
> standards both in scientific practice and personnel employed
>in the field.
>
>
> Richard Powell
> mailto:webmaster@hoslink.com
>
> http://www.hoslink.com/
>
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 00:32:27 -0500
> From: "Patrick M. Haley"
> Subject: Smooth muscle stainnng
>
> Dear Netters,
> I am staining animal tissues cow , cat, goat and want to
>increase the
> staining of the smooth muscle. I use 2% eosin y (alcoholic)
>for 2 min 30
> sec, 30 sec in 95% ethanol, then dehydrate to xylene.
>Nuclear stain is Gill
> III for 6 minutes.
> Any suggestions would be greatly appreciated.
>
> Also, will there be a histonet gathering at the NSH this
>year?
>
> Many thanks
>
> Pat
> HTN inc.
>
>
>
>
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 00:33:16 -0500
> From: "Amos Brooks"
> Subject: Re: CD117
>
> Hi,
> Use the Dako antibody! We get good results with no
>pretreatment at 1:200
> with LSAB+ detection. Use colon as a control as it is
>usually mast cell
> rich. Our results have been very reliable.
> Amos Brooks
>
> - ----- Original Message -----
> From: "Krahn, Daniel"
> To:
> Sent: Friday, July 27, 2001 11:03 AM
> Subject: CD117
>
>
> > Hi there,
> > Could anyone please help out with a protocol for running
>CD117? We
> > have been validating the Santa Cruz polyclonal (goat) and
>the Dako
> > polyclonal (rabbit) and are having some difficulties
>getting consistent
> > results.
> > A test protocol would be very helpful, including method of
>antigen
> > retrieval, dilution factors, instrumentation, etc.
> >
> > Thank you very much!!!!!!
> >
>
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 00:34:02 -0500
> From: SiskKidd@aol.com
> Subject: Re: LFB stain
>
>
> Hi Michelle,
> How long are you drying your slides? We have found
>that you need to
> leave the slides in a 37 degree oven overnight and then in a
>90 to 100 degree
> )C of course) for another hour.
> If this doesn't work, there are some red frosted
>slides that our IHC
> people use that I have used for LFBs also and those seem to
>work.
> Good luck and let me know how you do.
> Marley
>
>
>
> ******************* NOTE *******************
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> contained in the following MIME Information.
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>
> Hi
>Michelle,
>
How long are you
>drying your slides?
> We have found that you need to
>
leave the slides in a 37 degree oven overnight and then
>in a 90 to 100
> degree
>
)C of course) for another hour.
>
If this doesn't
>work, there are some
> red frosted slides that our IHC
>
people use that I have used for LFBs also and those seem
>to work.
>
Good luck and let
>me know how you do.
>
>
> &nbs
>p; &n
>bsp;
> &nbs
>p; Marley
>
> - --part1_9.18f27bc7.28938ef6_boundary--
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 00:41:35 -0500
> From: "J. A. Kiernan"
> Subject: Re: Microscopes in offices?
>
> On Fri, 27 Jul 2001 Snobird75@aol.com wrote:
> > We were unable to have our scope in our office when I
>worked at
> > the hospital lab. Due to people having drinks in the
>office.
>
> This is a genuinely grave safety matter. If you knock over
> a mug of coffee or a top-heavy paper cup of coke the liquid
> could get into the mechanical workings of the microscope -
> perhaps necessitating professional dissection and cleaning
> if the knobs become stiff as a result. Even worse, you
> might be using an objective as a magnifier to get a first
> impression of a section and then cough up come bits of a
> chewed up ham, lettuce and mustard sandwich with sesame
> seeds in the bread; and some of the expectoranda might
> go down the hole where the eyepiece was, falling perhaps
> even into the exit pupil of a plan-apo objective.
>
> The safety administrators should be venerated and praised
> for their wisdom, protecting their employers' expensive
> microscopes by ordering that they must be in labs, where
> nothing ever gets spilled (and even if it did it couldn't
> be harmful because all harmful substances were banned
> from labs years ago).
>
> If an accidentally dropped bottle of mounting medium in the
> lab were to be splash onto the objective, mechanical stage
> and condenser of a microscope it wouldn't matter at all.
> The well trained safety personnel would simply evacuate
> the lab for a day or two while the solvent evaporated
> and then let everyone back in to carry on with the work.
> The microscope would still work perfectly for examining
> the slide that was on its stage at the time of the
> accident.
>
> "Oh let us never, never doubt
> What nobody us sure about."
> Hillaire Belloc, 1898.
> - ----------------------------------------
> John A. Kiernan
> Department of Anatomy & Cell Biology
> The University of Western Ontario
> London, Canada N6A 5C1
> kiernan@uwo.ca
> http://publish.uwo.ca/~jkiernan
>
>
>
>
>
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 04:05:59 -0500
> From: "J. A. Kiernan"
> Subject: Re: sircam worm; subject headers; worthless emails
>
> On Fri, 27 Jul 2001 JHoffpa464@aol.com wrote:
>
> > ... Or is the histonet so unimportant that it doesn't
> > matter if you delete an email with no subject.
>
> Any email with no Subject is worthless and possibly
> malignant. Histonet doesn't come into it (though they
> might do well to exclude all improperly constituted
> submissions).
>
> If anyone has a genuine question to ask of a large
> group of people, he or she can put 4 or 5 words in
> the subject line to say what it's about. An enquirer
> who can't be bothered to do that doesn't deserve
> to be noticed.
>
> The subject line is the prime directive for hitting
> the Delete key. Unwanted ads and new viruses like
> the SIRCAM worm cash in on what they think are
> appealing Subjects. Anyone with an IQ > ?90 can
> spot the spam and the mischief, but that leaves
> some 3/4 of mankind as potential victims.
>
> - ----------------------------------------
> John A. Kiernan
> Department of Anatomy & Cell Biology
> The University of Western Ontario
> London, Canada N6A 5C1
> kiernan@uwo.ca
> http://publish.uwo.ca/~jkiernan
>
>
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 04:31:00 -0500
> From: Agustin Victor Chertcoff
> Subject: Argentine Society (for Peggy or Steven Slaps)
>
> Hi!
> I want communicate for the link to Argentine Society
> Histotechnologist is http://usuarios.tripod.es/SAH1 /
> this place is at this moment in construction
> Sorry.I wants request,please, it corrects in the Home Page
>Histotechs
>
> Thanks in advanced
> Agustin Chertcoff
>
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 04:33:35 -0500
> From: "Lee & Peggy Wenk"
> Subject: Re: "pink's disease"
>
> Do you mean all your tissue is too pink after staining with
>H&E?
>
> Try checking the pH of the eosin. Should be between 4-5.
> Adjust with acetic acid.
>
> More information on what stain and what exactly it looks
>like
> would be more helpful.
>
> Peggy A. Wenk, HLT(ASCP)
> William Beaumont Hospital
> Royal Oak, MI 48073
>
> - ----- Original Message -----
> From: "Heather Earp-Jones"
> To: "HISTONET"
> Sent: Friday, July 27, 2001 4:10 AM
> Subject: "pink's disease"
>
>
> > Hi
> > I am looking for information on pink's disease. One our
>lab's is
> > experiencing this problem which is affecting the quality
>of the staining.
> > What in your opinion causes it and what can be done to
>remedy the
> situation.
> > Any input would be welcome.
> > Thanks
> > H.Earp-Jones
> > SA
> >
> >
> >
>
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 07:58:52 -0500
> From: Robert Geske
> Subject: fat analysis
>
> we are currently engaged in quantitation of different types
>and anatomic
> locations of fat. we want to analyze the adipocytes in terms
>of their number
> per unit volume and the individual cells maximum diameter
>and from that
> infer cell volume. given that the cells maximum diameter
>can be up to 200
> microns, we want to sample slices of fat in excess of that
>number.
> we initially looked at 200 micron sections of fat frozen in
>OCT and
> sectioned in a cryostat. this was not adequate. our
>cryostat will section
> up to 300 microns in thickness and this is the next step.
>the sections are
> floated on a buffer and viewed at 4X with images being
>captured with a
> digital camera before analysis. we do not want to mount the
>sections on
> slides as this causes shape distortion (this was our first
>thought also ---
> section, mount, and ORO). are there any suggestions on
>equipment and/or
> techniques for cutting up to 500 micron sections of fixed or
>fresh fat.
> another possiblity i am looking into this weekend is
>sterological methods to
> determine the information we are after. we this, i believe,
>we will not
> have to take such thick sections. with the knowledge of the
>measurment of
> section thickness and area we should be able to analyze
>multiple planes
> through the section and make reasonable estimates about the
>population of
> adipocytes. does anyone have experience using this method
>for fat analysis?
>
> regards to all,
>
> rob
>
>
>***************************************************************************
> The contents of this communication are intended only for
>the addressee and
> may contain confidential and/or privileged material. If you
>are not the
> intended recipient, please do not read, copy, use or
>disclose this
> communication and notify the sender. Opinions, conclusions
>and other
> information in this communication that do not relate to the
>official
> business of my company shall be understood as neither given
>nor endorsed by
> it.
>
>***************************************************************************
>
>
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 09:48:48 -0500
> From: DDittus787@aol.com
> Subject: Re: CD117
>
>
> We use cd117 at a 1:100 dilution with a harsh microwave
>antigen retrieval
> using citrate, the a 32 min incubation at 42 degrees.
> Dana
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
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>------------------
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>
> - --part1_c.1924aae7.28942998_boundary
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>
> We use cd117
>at a 1:100
> dilution with a harsh microwave antigen retrieval
>
using citrate, the a 32 min incubation at 42 degrees.
>
>
> &nbs
>p; &n
>bsp;
> &nbs
>p; Dana
>
> - --part1_c.1924aae7.28942998_boundary--
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 19:58:23 -0500
> From: "Jeff and Wanda Gray"
> Subject: Testing, please delete
>
> Am I rid of the dreaded enclosures?
>
>
>
>
>
>
>----------------------------------------------------------------------
>
> Date: 28 Jul 2001 22:56:56 -0500
> From: Sunil Thomas K
> Subject: chrome-alum gelatin
>
> Hi,
> I have a question regarding chrome alum-gelatin used
> for subbing. It gets very viscous when referigerated
> (difficult to pour out from the glass bottle.
> Is this solution warmed before slides are dipped?
> should the slides be warmed in an oven so that
> adhesion is better?
>
> Sunil
>
> __________________________________________________
> Do You Yahoo!?
> Make international calls for as low as $.04/minute with
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>
>
>
>----------------------------------------------------------------------
>
> Date: 29 Jul 2001 00:10:31 -0500
> From: "J. A. Kiernan"
> Subject: Re: Molar ratio of Ca2+ for Alizarin stain
>
> On Fri, 27 Jul 2001, Montague, Donna C wrote:
> > To: 'Histonet'
> >
> > We are using Alizarin red stain as a colorimetric
>indicator of Ca++ in
> > ... Further, by looking up the chemical structure in
>Conn's, I surmise
> > the equivalence ratio would be 1 mole Alizarin per 2 moles
>Calcium.
> > Do you concur?
>
> No. The traditional tale is 2 alizarin to 1 calcium. With a
>coordination
> number of 4, a Ca2+ ion is supposed to bind to suitably
>spaced oxygens
> on 2 alizarin molecules, its charge being neutralized
>because on each
> alizarin molecule one of the oxygens is an ionized phenol
>group. BUT
> an in vitro study by Lievremont & 2 al (1982) Acta Anat.
>114:268-280
> found metal:dye approx= 1:1 in precipitates. A precipitate
>is not
> necessarily the same as a histochemical staining, where a
>large
> excess of the reagent is provided, followed by washing.
>
> Another consideration is that alizarin red S that you buy
>isn't
> 100% of that dye. The Biolog
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