I would like to know if anyone is having trouble with the writing coming off
of their cassettes. We are using Securline Marker 11/ Superfrost, from
Baxter. Somedays we open the processors and the writing is off on part off
the cassettes. One day we opened up the processor and about 12 cassettes had
no writing. We were denied a cassette labeler in this years budget. I was
wondering if anyone could share any tips on these pens are any other
suggestions.

S. Clark (sclark@mhs-net.com)

		-----Original Message-----
		From:	HistoNet Server
[mailto:histonet@pathology.swmed.edu]
		Sent:	Sunday, July 29, 2001 1:09 AM
		To:	HistoNet Server
		Subject:	Daily Digest


	
----------------------------------------------------------------------

		Date: 28 Jul 2001 00:30:43 -0500
		From: "Richard N Powell" 
		Subject: RE: Top Atomic Pathologists and OHS issues in US
labs

		Have to agree!

		Most US citizens don't seem to know that the big round thing
we live on has
		other countries - its amazing to see the number of US based
web sites that
		don't understand there is a big world out there with 60 to
70% more web
		users than there are in te US! Having said that, I have had
the pleasure of
		meeting several great American Pathologists - funny though
they don't seem
		to be any different than our local ones..........

		As to the thread on OHS issues surrounding flammables in
histo laboratories.
		Does the US have any uniform OHS Laws or are they all local
State laws?
		Surely your accreditation bodies also have a brief to look
at safety in
		laboratories?

		I guess that also begs the question of how many US labs are
actually
		accredited, have quality systems, ISO 9000 accreditation or
ISO/IEC 17025
		accreditation?

		My impression from these pages is that there is a huge
variation in
		standards both in scientific practice and personnel employed
in the field.


		Richard Powell
		mailto:webmaster@hoslink.com

		http://www.hoslink.com/



	
----------------------------------------------------------------------

		Date: 28 Jul 2001 00:32:27 -0500
		From: "Patrick M. Haley" 
		Subject: Smooth muscle stainnng

		Dear Netters,
		    I am staining animal tissues cow , cat, goat and want to
increase  the
		staining of the smooth muscle. I use 2% eosin y (alcoholic)
for 2 min 30
		sec, 30 sec in 95% ethanol, then dehydrate to xylene.
Nuclear stain is Gill
		III for 6 minutes.
		    Any suggestions would be greatly appreciated.

		Also, will there be a histonet gathering at the NSH this
year?

		Many thanks

		Pat
		HTN inc.






	
----------------------------------------------------------------------

		Date: 28 Jul 2001 00:33:16 -0500
		From: "Amos Brooks" 
		Subject: Re: CD117

		Hi,
		    Use the Dako antibody! We get good results with no
pretreatment at 1:200
		with LSAB+ detection. Use colon as a control as it is
usually mast cell
		rich. Our results have been very reliable.
		Amos Brooks

		- ----- Original Message -----
		From: "Krahn, Daniel" 
		To: 
		Sent: Friday, July 27, 2001 11:03 AM
		Subject: CD117


		> Hi there,
		> Could anyone please help out with a protocol for running
CD117?  We
		> have been validating the Santa Cruz polyclonal (goat) and
the Dako
		> polyclonal (rabbit) and are having some difficulties
getting consistent
		> results.
		> A test protocol would be very helpful, including method of
antigen
		> retrieval, dilution factors, instrumentation, etc.
		>
		> Thank you very much!!!!!!
		>



	
----------------------------------------------------------------------

		Date: 28 Jul 2001 00:34:02 -0500
		From: SiskKidd@aol.com
		Subject: Re: LFB stain


		  Hi Michelle,
		       How long are you drying your slides? We have found
that you need to 
		leave the slides in a 37 degree oven overnight and then in a
90 to 100 degree 
		)C of course) for another hour.
		       If this doesn't work, there are some red frosted
slides that our IHC 
		people use that I have used for LFBs also and those seem to
work.
		       Good luck and let me know how you do.
		                                                      Marley



		******************* NOTE *******************
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		contained in the following MIME Information.
		********************************************


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------------------


		- --part1_9.18f27bc7.28938ef6_boundary
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		  Hi
Michelle,
		
       How long are you
drying your slides?
		We have found that you need to 
		
leave the slides in a 37 degree oven overnight and then
in a 90 to 100
		degree 
		
)C of course) for another hour.
		
       If this doesn't
work, there are some
		red frosted slides that our IHC 
		
people use that I have used for LFBs also and those seem
to work.
		
       Good luck and let
me know how you do.
		

	
            &nbs
p;            &n
bsp;            
            &nbs
p;  Marley

		- --part1_9.18f27bc7.28938ef6_boundary--


	
----------------------------------------------------------------------

		Date: 28 Jul 2001 00:41:35 -0500
		From: "J. A. Kiernan" 
		Subject: Re: Microscopes in offices?

		On Fri, 27 Jul 2001 Snobird75@aol.com wrote:
		> We were unable to have our scope in our office when I
worked at 
		> the hospital lab. Due to people having drinks in the
office.

		This is a genuinely grave safety matter. If you knock over
		a mug of coffee or a top-heavy paper cup of coke the liquid
		could get into the mechanical workings of the microscope - 
		perhaps necessitating professional dissection and cleaning
		if the knobs become stiff as a result.  Even worse, you
		might be using an objective as a magnifier to get a first
		impression of a section and then cough up come bits of a
		chewed up ham, lettuce and mustard sandwich with sesame
		seeds in the bread; and some of the expectoranda might
		go down the hole where the eyepiece was, falling perhaps 
		even into the exit pupil of a plan-apo objective.

		The safety administrators should be venerated and praised
		for their wisdom, protecting their employers' expensive
		microscopes by ordering that they must be in labs, where 
		nothing ever gets spilled (and even if it did it couldn't
		be harmful because all harmful substances were banned
		from labs years ago). 

		If an accidentally dropped bottle of mounting medium in the
		lab were to be splash onto the objective, mechanical stage 
		and condenser of a microscope it wouldn't matter at all.
		The well trained safety personnel would simply evacuate
		the lab for a day or two while the solvent evaporated
		and then let everyone back in to carry on with the work.
		The microscope would still work perfectly for examining
		the slide that was on its stage at the time of the
		accident. 

		"Oh let us never, never doubt
		 What nobody us sure about."
		                              Hillaire Belloc, 1898.
		- ----------------------------------------
		John A. Kiernan
		Department of Anatomy & Cell Biology
		The University of Western Ontario
		London,  Canada   N6A 5C1
		   kiernan@uwo.ca
		   http://publish.uwo.ca/~jkiernan
		 


		 



	
----------------------------------------------------------------------

		Date: 28 Jul 2001 04:05:59 -0500
		From: "J. A. Kiernan" 
		Subject: Re: sircam worm; subject headers; worthless emails 

		On Fri, 27 Jul 2001 JHoffpa464@aol.com wrote:

		> ...   Or is the histonet so unimportant that it doesn't 
		> matter if you delete an email with no subject.

		Any email with no Subject is worthless and possibly
		malignant. Histonet doesn't come into it (though they
		might do well to exclude all improperly constituted
		submissions).

		If anyone has a genuine question to ask of a large
		group of people, he or she can put 4 or 5 words in
		the subject line to say what it's about. An enquirer
		who can't be bothered to do that doesn't deserve
		to be noticed.

		The subject line is the prime directive for hitting
		the Delete key. Unwanted ads and new viruses like 
		the SIRCAM worm cash in on what they think are
		appealing Subjects. Anyone with an IQ > ?90 can
		spot the spam and the mischief, but that leaves
		some 3/4 of mankind as potential victims.
		 
		- ----------------------------------------
		John A. Kiernan
		Department of Anatomy & Cell Biology
		The University of Western Ontario
		London,  Canada   N6A 5C1
		   kiernan@uwo.ca
		   http://publish.uwo.ca/~jkiernan
		 



	
----------------------------------------------------------------------

		Date: 28 Jul 2001 04:31:00 -0500
		From: Agustin Victor Chertcoff 
		Subject: Argentine Society (for Peggy or Steven Slaps) 

		Hi!
		I want  communicate  for the  link to Argentine Society
		Histotechnologist is http://usuarios.tripod.es/SAH1 /
		this place is at this moment in construction
		Sorry.I wants request,please, it corrects in the Home Page
Histotechs

		Thanks in advanced
		Agustin Chertcoff 
		Subject: Re: "pink's disease"

		Do you mean all your tissue is too pink after staining with
H&E?

		Try checking the pH of the eosin. Should be between 4-5.
		Adjust with acetic acid.

		More information on what stain and what exactly it looks
like
		would be more helpful.

		Peggy A. Wenk, HLT(ASCP)
		William Beaumont Hospital
		Royal Oak, MI 48073

		- ----- Original Message -----
		From: "Heather Earp-Jones" 
		To: "HISTONET" 
		Sent: Friday, July 27, 2001 4:10 AM
		Subject: "pink's disease"


		> Hi
		> I am looking for information on pink's disease. One our
lab's is
		> experiencing this problem which is affecting the quality
of the staining.
		> What in your opinion causes it and what can be done to
remedy the
		situation.
		> Any input would be welcome.
		> Thanks
		> H.Earp-Jones
		> SA
		>
		>
		>



	
----------------------------------------------------------------------

		Date: 28 Jul 2001 07:58:52 -0500
		From: Robert Geske 
		Subject: fat analysis

		we are currently engaged in quantitation of different types
and anatomic
		locations of fat. we want to analyze the adipocytes in terms
of their number
		per unit volume and the individual cells maximum diameter
and from that
		infer cell volume.  given that the cells maximum diameter
can be up to 200
		microns, we want to sample slices of fat in excess of that
number.
		we initially looked at 200 micron sections of fat frozen in
OCT and
		sectioned in a cryostat.  this was not adequate.  our
cryostat will section
		up to 300 microns in thickness and this is the next step.
the sections are
		floated on a buffer and viewed at 4X with images being
captured with a
		digital camera before analysis. we do not want to mount the
sections on
		slides as this causes shape distortion (this was our first
thought also ---
		section, mount, and ORO).  are there any suggestions on
equipment and/or
		techniques for cutting up to 500 micron sections of fixed or
fresh fat.
		another possiblity i am looking into this weekend is
sterological methods to
		determine the information we are after.  we this, i believe,
we will not
		have to take such thick sections.  with the knowledge of the
measurment of
		section thickness and area we should be able to analyze
multiple planes
		through the section and make reasonable estimates about the
population of
		adipocytes.  does anyone have experience using this method
for fat analysis?

		regards to all,

		rob

	
*************************************************************************** 
		 The contents of this communication are intended only for
the addressee and
		may contain confidential and/or privileged material. If you
are not the
		intended recipient, please do not read, copy, use or
disclose this
		communication and notify the sender.  Opinions, conclusions
and other
		information in this communication that do not relate to the
official
		business of my company shall be understood as neither given
nor endorsed by
		it.  
	
*************************************************************************** 




	
----------------------------------------------------------------------

		Date: 28 Jul 2001 09:48:48 -0500
		From: DDittus787@aol.com
		Subject: Re: CD117


		We use cd117 at a 1:100 dilution with a harsh microwave
antigen retrieval 
		using citrate, the a 32 min incubation at 42 degrees.
		                                                     Dana



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		We use cd117
at a 1:100
		dilution with a harsh microwave antigen retrieval 
		
using citrate, the a 32 min incubation at 42 degrees.
		

	
            &nbs
p;            &n
bsp;            
            &nbs
p; Dana

		- --part1_c.1924aae7.28942998_boundary--


	
----------------------------------------------------------------------

		Date: 28 Jul 2001 19:58:23 -0500
		From: "Jeff and Wanda Gray" 
		Subject: Testing, please delete

		Am I rid of the dreaded enclosures?





	
----------------------------------------------------------------------

		Date: 28 Jul 2001 22:56:56 -0500
		From: Sunil Thomas K 
		Subject: chrome-alum gelatin

		Hi,
		I have a question regarding chrome alum-gelatin used
		for subbing. It gets very viscous when referigerated
		(difficult to pour out from the glass bottle. 
		Is this solution warmed before slides are dipped?
		should the slides be warmed in an oven so that
		adhesion is better?  

		Sunil

		__________________________________________________
		Do You Yahoo!?
		Make international calls for as low as $.04/minute with
Yahoo! Messenger
		http://phonecard.yahoo.com/


	
----------------------------------------------------------------------

		Date: 29 Jul 2001 00:10:31 -0500
		From: "J. A. Kiernan" 
		Subject: Re: Molar ratio of Ca2+ for Alizarin stain

		On Fri, 27 Jul 2001, Montague, Donna C wrote:
		> To: 'Histonet' 
		> 
		> We are using Alizarin red stain as a colorimetric
indicator of Ca++ in
		> ... Further, by looking up the chemical structure in
Conn's, I surmise 
		> the equivalence ratio would be 1 mole Alizarin per 2 moles
Calcium. 
		> Do you concur?

		No. The traditional tale is 2 alizarin to 1 calcium. With a
coordination
		number of 4, a Ca2+ ion is supposed to bind to suitably
spaced oxygens
		on 2 alizarin molecules, its charge being neutralized
because on each
		alizarin molecule one of the oxygens is an ionized phenol
group. BUT
		an in vitro study by Lievremont & 2 al (1982) Acta Anat.
114:268-280
		found metal:dye approx= 1:1 in precipitates. A precipitate
is not
		necessarily the same as a histochemical staining, where a
large
		excess o