NaOH soaking of tissue

From:"Sebree Linda A."

A mini survey histonetters:

Do you soak any/all FFPE tissues in dilute NaOH solutions prior to
sectioning in order to facilitate sectioning?

How long?

Only specific tissues?

What strength of NaOH?

We stopped doing this several years ago due to possible problems with
immunohistochemical staining.  Do any of you "soakers" notice problems in
your IHC staining?

Thanks for the input,

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792-

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