Hello Histonetters!

I am having a problem with a Luxol Fast Blue Stain that I am doing on a
section of brain for the HTL registry. I have run the stain several times
and every time the sections have washed off of the slides. I have tried
using gelatin, poly-l-lysine, and even Elmer's glue in the water bath to
remedy this problem.

The section stayed on once when I used a double coating of Poly, but then
the stain came out very uneven and there was a deep blue staining where the
poly had been. Does anyone have any suggestions? The deadline for the
registry is looming! Thanks in advance.

Michelle Rederick  HT(ASCP)
Sharon Regional Health System
740 East