I am having a problem with a Luxol Fast Blue Stain that I am doing on a
section of brain for the HTL registry. I have run the stain several times
and every time the sections have washed off of the slides. I have tried
using gelatin, poly-l-lysine, and even Elmer's glue in the water bath to
remedy this problem.
The section stayed on once when I used a double coating of Poly, but then
the stain came out very uneven and there was a deep blue staining where the
poly had been. Does anyone have any suggestions? The deadline for the
registry is looming! Thanks in advance.
Michelle Rederick HT(ASCP)
Sharon Regional Health System
<< Previous Message | Next Message >>