LCM and antigen retrieval

From:Denise Bland-Piontek

Is anyone out there doing Laser Capture Microdissection on
Immunoperoxidase stains that require antigen retrieval? We are using a
double stain procedure with CD-68 and CD-34 (DAKO products), marking
with DAB and Fast Red. We are using antigen retrieval and charged
slides. We realize that charged slides are not recommended for LCM,
however we do not want to loose the tissue during antigen retrieval.
Others have somehow gotten this to work and I was wondering if anyone
has any tricks they can share with me. Thanks in advance,
Denise Bland-Piontek, HTL
University of Minnesota

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