I have read that this is possible using the extremely
sensitive tyramide signal amplification (TSA) system.
The idea is that you first stain with one antibody
at such a high dilution that it can only be visualized
by TSA. Then you do a conventional stain with the other.
I haven't done this myself, but it has been described at
I think I've seen some publications also, so you could
try a Medline search.
You'll find information on the TSA system itself at
Mikael Niku URL: www.helsinki.fi/~mniku/
University of Helsinki Dept. Basic Veterinary Sciences
- Mit=E4k=F6 mielt=E4 olen l=E4nsimaisesta sivistyksest=E4?
Minusta se olisi erinomainen ajatus!
----- Original Message -----
From: "Bruce Abaloz" <firstname.lastname@example.org>
Sent: Thursday, July 19, 2001 3:33 AM
Subject: IHC Double staining
> I am a student in the department of Zoology at the University of
Melbourne, Australia. I have two antibodies raised in the same species
however I need to co-localize their expression in the one tissue section.
I have protocols for immuno double staining of antibodies raised in
different species, but I can not find a protocol or a kit that would allo=
me to achieve this with two antibodies raised in the same species. I am
currently using immunofluorescence and would like to remain with this
visualization. I would be very grateful for any help.
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