Fwd: LFB staining

From:Christina Benou <cbenou@rics.bwh.harvard.edu>

>Hi everyone,
>I am trying to stain mouse brain with Luxol Fast Blue. I perfused 
>the mouse with 4%paraformaldehyde, then I put the brain in PFA for 3 
>days and then in 30% sucrose for 1 day. I froze the tissue in OCT at 
>-20 degrees. My problem is that the sections disappear from the 
>slide during the overnight LFB immersion. Does anyone have any idea 
>about why this is happening?

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