Bouins and immunos

From:Elizaha@aol.com


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Hi,
    Has anyone been able to do immunos with Bouins fixed tissue?
    
     I have tried with two antibodies against  Androgen Receptor and inhibin 
and both don't work, at least not for me.  (EDTA and Citrate antigen 
retrieval, trypsin and Prot K digestion were tried)
     
     I am trying to convince the boss that Formalin fixation is probably an 
answer but in the meantime there is still  alot of tissue samples fixed in 
Bouins so......maybe someone knows something to try with Bouins that I 
didn't..  (I looked in the archives but it only mentioned in situ and Bouins, 
specifically).

Thanks for any help,
Elizabeth

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Hi,

   Has anyone been able to do immunos with Bouins fixed tissue?
   
    I have tried with two antibodies against  Androgen Receptor and inhibin
and both don't work, at least not for me.  (EDTA and Citrate antigen
retrieval, trypsin and Prot K digestion were tried)
    
    I am trying to convince the boss that Formalin fixation is probably an
answer but in the meantime there is still  alot of tissue samples fixed in
Bouins so......maybe someone knows something to try with Bouins that I
didn't..  (I looked in the archives but it only mentioned in situ and Bouins,
specifically).

Thanks for any help,
Elizabeth

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