Acid Alcohol Fixation & Plastics?
|From:||Tracey Couse <firstname.lastname@example.org>|
We are looking to bring plastics to our facility as a large part of our
research involves bone and other hard tissues. The HistoNet has been
exceptionally helpful thus far in providing information and references for
this type of work. Thank you! We have an upcoming project where
immunohistochemistry will be performed using an antibody where the vendor
suggests acid alcohol fixation. We need to plastic embed the
tissue. Based on my research to date, formalin seems to be the fixative of
choice for plastic work but, previous work done using this antibody on
formalin fixed tissue yielded negative results. Is acid alcohol an option
for tissue to be plastic (methyl methacrylate) embedded?
Thanks in advance for any help.
Petit Institute for Bioengineering & Biosciences
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