Acid Alcohol Fixation & Plastics?

From:Tracey Couse <>

Hello HistoNet!	

We are looking to bring plastics to our facility as a large part of our 
research involves bone and other hard tissues. The HistoNet has been 
exceptionally helpful thus far in providing information and references for 
this type of work.  Thank you!  We have an upcoming project where 
immunohistochemistry will be performed using an antibody where the vendor 
suggests acid alcohol fixation.  We need to plastic embed the 
tissue.  Based on my research to date, formalin seems to be the fixative of 
choice for plastic work but, previous work done using this antibody on 
formalin fixed tissue yielded negative results.  Is acid alcohol an option 
for tissue to be plastic (methyl methacrylate) embedded?

Thanks in advance for any help.

Tracey Couse
Petit Institute for Bioengineering & Biosciences
Georgia Inst

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