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I have not done any work with these particular receptors  but have used
Bouin's fixed tissues for others. Most of this was done before antigen
retrieval evolved so that antibodies were used directly. Our best
results were using Bouin's fixation. All the steps from fixation up to
the intermediary agent wer carried out in the refrigerator. In some work
I did a long time ago using lectins (which  have many similarities to
antibodies in their binding properties), I found that when Bouin's was
used on frozen sections, the lectin binding was usually very poor. If
however the lectin was applied to sections of tissues that had been
fixed in Bouin's and then processed to paraffin wax, that the
specificity of the binding was significantly improved.
It is assumed that there is some unmasking as tissues go t

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