re:antigen retrieval

From:Schmang155@aol.com


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We uses Trilogy for antigen retrieval or cell conditioning--whatever you wnt 
to call it. Has anyone had any success with reusing this solution for antigen 
retrieval? We use two containers in a steamer for 30 min each. We discard the 
first solution and only reuse the second if another antigen retrieval  is 
requested on that same day. Trilogy is expensive though and it would be nice 
to conserve solution if we could.  Thanks----------Angie Schmidt
                                                                           
Tri-Health Labs
                                                                          
Good Samaritan Hosp
                                                                          
Cincinnati, Ohio

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<HTML><FONT FACE=arial,helvetica><FONT  COLOR="#ff0000" SIZE=2 FAMILY="SCRIPT" FACE="Comic Sans MS" LANG="0">We uses Trilogy for antigen retrieval or cell conditioning--whatever you wnt 
<BR>to call it. Has anyone had any success with reusing this solution for antigen 
<BR>retrieval? We use two containers in a steamer for 30 min each. We discard the 
<BR>first solution and only reuse the second if another antigen retrieval  is 
<BR>requested on that same day. Trilogy is expensive though and it would be nice 
<BR>to conserve solution if we could.  Thanks----------Angie Schmidt
<BR>                                                                           
<BR>Tri-Health Labs
<BR>                                                                          
<BR>Good Samaritan Hosp
<BR>                                                                          
<BR>Cincinnati, Ohio</FONT></HTML>

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