osmium for autofluorescence.
|From:||Debra Dunlap <dydunlap@gene.COM>|
I am currently using osmium tetroxide to reduce autofluorescence. I
have had luck using fast red but am still struggling using fluorescent
probes. The osmium seems to be quenching the fluorescent probe signal
as well. I think Dr. Kiernans suggestion of rinsing overnight should
help alot. I have only been rinsing for 30 min. What am I actually
rinsing away? Is is free osmium tetroxide or am I hydrolyzing the
cyclic osmic ester? Any help is greatly appreciated. I have been
struggling with this problem for awhile.
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