froozen renals with shandon cryostat

From:Schmang155@aol.com


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First of all I want to thank everyone for their renal help so far--We are to 
be up and running by the 23 but I still have a few hurdles left.  We have a 
Shandon cryostat which is never used that I was thinking of using for our 
renal biopsies. 
Is anyone out there using a shandon currently and, if so, what kind of 
procedure do you follow.  I can use an old Miles cryostat but the Shandon is 
newer and will hold up longer.
    Also thanks for the many answers on antigen retreival. We are using 
Trilogy because we can skip deparaffination with it. We will try the many 
suggestions on using it more than once.
                                                               Angie 
Schmidt---Tri-Health
                                                                 Cincinnati, 
Ohio

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<HTML><FONT FACE=arial,helvetica><FONT  COLOR="#000080" SIZE=2 FAMILY="SCRIPT" FACE="Comic Sans MS" LANG="0">First of all I want to thank everyone for their renal help so far--We are to 
<BR>be up and running by the 23 but I still have a few hurdles left.  We have a 
<BR>Shandon cryostat which is never used that I was thinking of using for our 
<BR>renal biopsies. 
<BR>Is anyone out there using a shandon currently and, if so, what kind of 
<BR>procedure do you follow.  I can use an old Miles cryostat but the Shandon is 
<BR>newer and will hold up longer.
<BR>    Also thanks for the many answers on antigen retreival. We are using 
<BR>Trilogy because we can skip deparaffination with it. We will try the many 
<BR>suggestions on using it more than once.
<BR>                                                               Angie 
<BR>Schmidt---Tri-Health
<BR>                                                                 Cincinnati, 
<BR>Ohio</

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