Me again,

I am having an awful time with these two stains, would some wise person tell 
me what I am doing wrong?  I am using FFPE spleen as a control.  I seems to 
stain wheither I use primary antibody or not.  I have tried blocking with 
serum-free and also 10% normal serum with 5% BSA in buffer.  I am using Dako's 
Envision plus mouse kit (HRP).  I block with their peroxidase blocking 
solution.  Any ideas?

Thanks,
Kathy

Kathy Walters                                // 
Central Microscopy Research Facility        / /
85 EMRB                                    / /\
University of Iowa                        / /\ \
Iowa City, Iowa 52242                    / /  \ \
Phone #:  (319) 335-8142                / /    \ \
Fax #:  (319) 384-4469                ______   ((0))
email: Katherine-Walters@uiowa.edu     |__|    / /
                                        ||    / /
                                     --------------
                                   ------------------