Reusing of retrieval solution

From:Tom Wells <>

I was wondering whether or not people reuse retrieval solution after HIER
and if you do how many times do you reuse it. We currently use Dako's
retrieval solution and discard it after each use, but, it seems to me that
there is no chemical reason to discard it. Any comments would be
appreciated. Thanks. Tom

Tom Wells, BSc, ART
Supervisor, Immunohistochemistry Laboratory
Lions Gate Hospital
North Vancouver, BC

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