Retic trouble
From: | Linda Stirpe <lpstirpe@yahoo.com> |
Hi Fellow Histonetters,
I need some help trouble shooting Modified Gomoris
Retic and could not think of a better place to ask for
help. Lately, the control and/or sections are
floating when going from the silver solution to
distilled water and sometimes when going from
distilled water to 20% formalin. The sections are cut
at 5 microns and put on Poly-L-Lysine slides. We have
tried drying the slides differently each time - slide
dryer, 37 degree incubator overnight and 57 degree
incubator for 1 hour. We have made up all new
reagents and the sections still float. All the
reagents and steps done at room temp. I do not know
what else to try, so, any suggestions are greatly
appreciated.
Thanks in advance,
Linda Stirpe
Corning Hospital
Corning, NY
__________________________________________________
Do You Yahoo!?
Get personalized email addresses from Ya
<< Previous Message | Next Message >>