Re: stain for platelet & fibrin

From:Renee Escalona <>


Our lab uses Carstairs Method for fibrin and platelets.  Here it is:

Fixation:  NBF  (formalin saline recommended)

Sections: 5 microns


Picric acid-Orange G Solution:       20 ml  saturated picric acid aqueous
                                                      80 ml   saturated picric acid in isopropanol
                                                       0.2 gm  Orange G

Ponceau-Fuchsin Solution:        0.5 gm   acid fuchsin
                                                  0.5 gm    Ponceau 2R
                                                   1 ml   glacial acetic acid
                                                   99 ml   distilled water

Aniline Blue Solution:            1 gm   aniline blue
                                             100 ml   1% acetic acid


1.   Deparaffinize and hydrate to water.
2.   Mordant in 5% ferric alum for 5 minutes and then rinse in tap water.
3.   Stain with Mayer's Hematoxylin for 5 minutes and rinse in tap water.
4.   Stain in picric acid-Orange G solution for 30 minutes to 1  hour.
5.   Rinse once in distilled water.
6.   Differentiate with 1% phosphotungstic acid.
7.   Stain with aniline blue solution for 30 minutes to 2 hours.
8.   Rinse in several changes of distilled water.
9.   Dehydrate, clear and mount. 


fibrin - bright red                                        muscle - red
platelets - gray-blue to navy                      RBC's - clear yellow
collagen - bright blue

from Theory & Practice of Histotechnology, Second Edition, page 281

Good luck Cathy!

Renee Escalona, BS, HTL(ASCP)
Laboratory Supervisor
Texas Veterinary Medical Diagnostic Lab
College Station, TX

>>> Cathy Mayton <> 06/28/01 02:23PM >>>
Fellow Histonetters,

We are looking for stain specific for platelets and fibrin.  The tissues
are iliac or femoral arteries that will be embedded in paraffin.  Is the a
special stain specific to platelets and fibrin or is there an IHC method
that would be more specific?

Thanks in advance,

GLP Compliant Laboratory

Cathy A. Mayton
Project Director
Wasatch Histo Consultants, Inc. 

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