Re: stain for platelet & fibrin
|From:||Renee Escalona <firstname.lastname@example.org>|
Our lab uses Carstairs Method for fibrin and platelets. Here it is:
Fixation: NBF (formalin saline recommended)
Sections: 5 microns
Picric acid-Orange G Solution: 20 ml saturated picric acid aqueous
80 ml saturated picric acid in isopropanol
0.2 gm Orange G
Ponceau-Fuchsin Solution: 0.5 gm acid fuchsin
0.5 gm Ponceau 2R
1 ml glacial acetic acid
99 ml distilled water
Aniline Blue Solution: 1 gm aniline blue
100 ml 1% acetic acid
1. Deparaffinize and hydrate to water.
2. Mordant in 5% ferric alum for 5 minutes and then rinse in tap water.
3. Stain with Mayer's Hematoxylin for 5 minutes and rinse in tap water.
4. Stain in picric acid-Orange G solution for 30 minutes to 1 hour.
5. Rinse once in distilled water.
6. Differentiate with 1% phosphotungstic acid.
7. Stain with aniline blue solution for 30 minutes to 2 hours.
8. Rinse in several changes of distilled water.
9. Dehydrate, clear and mount.
fibrin - bright red muscle - red
platelets - gray-blue to navy RBC's - clear yellow
collagen - bright blue
from Theory & Practice of Histotechnology, Second Edition, page 281
Good luck Cathy!
Renee Escalona, BS, HTL(ASCP)
Texas Veterinary Medical Diagnostic Lab
College Station, TX
>>> Cathy Mayton <Cathy@wasatchhisto.com> 06/28/01 02:23PM >>>
We are looking for stain specific for platelets and fibrin. The tissues
are iliac or femoral arteries that will be embedded in paraffin. Is the a
special stain specific to platelets and fibrin or is there an IHC method
that would be more specific?
Thanks in advance,
GLP Compliant Laboratory
Cathy A. Mayton
Wasatch Histo Consultants, Inc.
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