Re: stain for platelet & fibrin
From: | Renee Escalona <rescalona@tvmdl.tamu.edu> |
Cathy,
Our lab uses Carstairs Method for fibrin and platelets. Here it is:
Fixation: NBF (formalin saline recommended)
Sections: 5 microns
Solutions:
Picric acid-Orange G Solution: 20 ml saturated picric acid aqueous
80 ml saturated picric acid in isopropanol
0.2 gm Orange G
Ponceau-Fuchsin Solution: 0.5 gm acid fuchsin
0.5 gm Ponceau 2R
1 ml glacial acetic acid
99 ml distilled water
Aniline Blue Solution: 1 gm aniline blue
100 ml 1% acetic acid
Procedure:
1. Deparaffinize and hydrate to water.
2. Mordant in 5% ferric alum for 5 minutes and then rinse in tap water.
3. Stain with Mayer's Hematoxylin for 5 minutes and rinse in tap water.
4. Stain in picric acid-Orange G solution for 30 minutes to 1 hour.
5. Rinse once in distilled water.
6. Differentiate with 1% phosphotungstic acid.
7. Stain with aniline blue solution for 30 minutes to 2 hours.
8. Rinse in several changes of distilled water.
9. Dehydrate, clear and mount.
Results:
fibrin - bright red muscle - red
platelets - gray-blue to navy RBC's - clear yellow
collagen - bright blue
from Theory & Practice of Histotechnology, Second Edition, page 281
Good luck Cathy!
Renee Escalona, BS, HTL(ASCP)
Laboratory Supervisor
Texas Veterinary Medical Diagnostic Lab
College Station, TX
>>> Cathy Mayton <Cathy@wasatchhisto.com> 06/28/01 02:23PM >>>
Fellow Histonetters,
We are looking for stain specific for platelets and fibrin. The tissues
are iliac or femoral arteries that will be embedded in paraffin. Is the a
special stain specific to platelets and fibrin or is there an IHC method
that would be more specific?
Thanks in advance,
Cathy
*********************************
GLP Compliant Laboratory
Cathy A. Mayton
Project Director
Wasatch Histo Consultants, Inc.
www.wasatchhisto.com
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