Re: osmium tetroxide-immunoreactivity
From: | "J. A. Kiernan" <jkiernan@uwo.ca> |
On Mon, 9 Jul 2001, Rudolf Schicho wrote:
> I've just read Dr. Kiernans answer to Debra Dunlap about osmium
> tetroxide for autofluorescence. Can osmium tetroxide treatment damage
> the immunoreactivity of your target protein?
Yes, that's a possibility. Traditional fixation in OsO4
(or post-osmication for that matter) greatly reduces affinity
for anionic dyes, presumably by bringing about oxidative
deamination of lysine side-chains. I don't think this
happens on a large scale with the milder treatment applied
to paraffin sections, but it would be sensible to compare
the appearances in sections that have & have not received
the OsO4 treatment and washing.
----------------------------------------
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan
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