Re: osmium references
|From:||"J. A. Kiernan" <email@example.com>|
Here are 3 references.
On Mon, 9 Jul 2001, Debra Dunlap wrote:
> when I meant 'fluorochrome" in my original message. I am certainly
> interested in the references that you mentioned. Thank you again,
The earliest use of OsO4 to quench autofluorescence (that
I know about) is:
Ornstein L, Mautner W, Davis BJ, Tamura R: New horizons in
fluorescence microscopy. Journal of the Mount Sinai Hospital
They used 2% OsO4 with only a brief wash, but they were
using a tremendously powerful fluorochrome. I've done their
method (a fluorescent PAS variant, using acriflavine); you
can see some photomicrographs in J. Anat. 99:926-928.
For use of weaker OsO4 solutions and longer washes:
Stoddart RW, Kiernan JA: Histochemical detection of the
`-D-arabinopyranoside configuration using fluorescent-labelled
concanavalin A. Histochemie 33:87-94, 1973.
[The ` in the title should be the greek letter alpha.]
Kiernan JA, Stoddart RW: Fluorescent-labelled aprotinin: a
new reagent for the histochemical detection of acid
mucosubstances. Histochemie 34:77-84, 1973.
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
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