What tissue types are you experiencing this non-specific staining on?  And
what detection method are you using (Avidin-biotin or other)?  Need more
info.  Jan

----- Original Message -----
From: "Martha Ward" <mward@wfubmc.edu>
To: <histonet@pathology.swmed.edu>
Sent: Friday, July 13, 2001 10:40 AM
Subject: non-specific staining


> Martha Ward writes:
>
> We are experiencing non-specific staining on our negative control slides
> in some cases where we must use EDTA, pH 8 for our antigen retrieval
> solution.  We do not see this with every tissue and I haven't been able
> to pin down exactly why it is happening.  We use a Ventana ES for our
> staining and I was wondering if anyone has experienced this and what can
> I do to eliminate it.  Than