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<P>Hello everybody,</P>
<P>Having recently joined this list server (yesterday), it appears that this may be an appropriate medium to pose questions as they pertain to histology and histotechiques. As a novice, I have started working with projects that require knowledge on staining, preparation of tissue (perfusion, embedding) specifically for collagen deposition. </P>
<P>I would like to ask all of the <STRONG>gurus </STRONG>here about Masson's Trichrome staining. I am following the Armed Forces protocol on this staining. Previous staining appeared to red (perhaps I did not wash the Biebrich scarlet acid fushin enough) to my eyes. While ameliorating it with better washing time, it seems now that the aniline blue is too blue as well. Interestingly, during the procedure of placing the slides in the coplin jar, it appeared as though after 5 min, the aniline was faintly stained in the tissue. This led me to repeat this process for another 2 times. Now, I wonder if repeating the aniline twice was just inappropriate and that although the aniline may seen faint on the slides, it's actually OK.</P>
<P>For those that use masson's trichrome, I would love to exchange procedures and hear your observations.</P>
<P>Thank you for your attention,</P>
<P>Eliane Valente</P>
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