Re: cold or warm pfa (also: what is PFA ?)

From:"J. A. Kiernan" <>

On Wed, 11 Jul 2001, Sunil Thomas K wrote:
> for transcardial perfusion what is generally used. cold
> or warm ( body temprature) PFA. I have seen reports
> which specifically state they used cold PFA (4oC).

Think!  What might be better than room temp (20C)
and WHY ???  (I assume that the fixative is formaldehyde.)

Cold will constrict the blood vessels.
Warm will accelerate autolysis.

What is "PFA" ? 

John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London,  Canada   N6A 5C1

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