Date sent:      	Fri, 06 Jul 2001 10:58:25 +0200
From:           	Paul Klosen <klosen@neurochem.u-strasbg.fr>
Subject:        	c-fos antibodies
To:             	histonet@pathology.swmed.edu

> Hi,
> 
> We are currently trying to set up a series of experiments with c-fos 
> detection on formalin fixed paraffin embedded sections. The antibody we 
> were using so far on vibratome sections does not seem to work on FFPE 
> tissue. 

Hi Paul, I am currently using c-fos(AB-2) from Oncogene Research 
Products. The research is looking at circadian rhythms in mice and 
its expression in the supra chiasmic nucleus(SCN).It appears to be 
working very nicely on FFPE tissue with proteolytic digestion, 
although I have yet to demonstrate this in the SCN (detects myelin 
e.g. optic chiasma, corpus callosum,red nucleus).
You may be using it as a cell growth/tumour marker so a more 
appropriate control may be required (breast or colonic adenoCa.)
If you require more information about the procedure/work/antibody, 
then e-mail me direct.
Terry.
Terry Hacker,
Medical Research Council,
Harwell,
Didcot,
Oxfordshire, OX11 ORD
01235 834393 x360