Re: background on CD20 & CD21 stained slides

From:Patti Loykasek <>

If you are using Envision+ for detection, the background can't be from
biotin. Have you tried titering out your antibodies? I have found Envision
to be a very clean detection system, and usually no need for even a serum
block. Take one of your antibodies, and do some doubling titers with it.
What are you using for a diluent? Also, what did you use in place of the
primary when you used the spleen without either antibody? How are you
rinsing in between steps of your procedure? Both of those antibodies can
usually be used with very little background problems. If I think of anything
else, I'll send another post.

Patti Loykasek
Phenopath Laboratories
Seattle, WA 

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