Hi,

I've posted this several times before here.  The best method I've found for repairing these is to cut around the tissue, through the coverslip, with a scapel blade, then coverslip with regular mounting media and a glass coverslip.  This will even work where the coverslip has broken the tissue and left some on the slide and some on the 'slip.  Just carefully position the cut coverslip in a little media in the area where the remaining tissue is, then coverslip as above.  You can reposition slightly under here by moving the glass coverslip.

Whew!!!

Good Luck
Aidan


__

aidan schurr  b.m.l.sc
section head, histology
hutt valley district health board
lower hutt
new zealand

aidan.schurr@hvh.co.nz
++64 4 570 9173

>>> "Connelly.J. Keith" <connelly@niehs.nih.gov> 6:07:09 a.m. Wednesday, 4 July 2001 >>>
Fellow Histonetters,
Our Facility has a large number of H & E tissue slides that have
plastic "film" coverslips.  In a number of instances the tissues are
actually adhearing to the coverslip and are coming completely off the
slide.  One Histology Correspondent suggested it might not be
appropriate to use Xylene to repair these because it might liquify the
plastic film coverslip.  She also wasn't sure if Permount mounting
media would liquify the plastic film coverslip.  Any insight that
could be provided into successful resolution of this dilemma would be
greatly appreciated.
Sincerely,
J. Keith Connelly