Re: Pap or MGG?
From: | =?iso-8859-1?q?Steve=20Machin=20UK?= <stevemachinuk@yahoo.co.uk> |
Steve Machin Uk replies
Try washing the slides in warm to hot water to remove the EA50 and
Orange G then in acid alcohol to get the Haematoxylin out.
N.b. Warm water works well for Eosin and Trichrome stains too and
doesn't take out the Haematoxylin.
--- WWmn916@aol.com wrote: > Greetings,
> The scenario was this: Slides that were meant to be stained
> MGG were
> accidentally stained Pap stain. What would other histotech's do to
> handle
> this situation? Destain/restain with acid alcohol does not work.
> Can Pap's
> be destained in other ways or would it simply be better to stain
> over the
> slides with mgg? Thanks for any input.
>
> Deb
>
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