Re: NBT/BCIP

From:Agripina Suarez <ASuarez@mrl.ubc.ca>

Hi,
I have used this chromogen for many years now, always dehydrate 
with alcohol and clear with xylene with no problem at all -- never 
seen any fading.
Agripina


Date sent:      	Thu, 12 Jul 2001 11:22:58 -0700 (PDT)
From:           	James Mubiru <jamubiru@yahoo.com>
Subject:        	NBT/BCIP
To:             	histonet@pathology.swmed.edu

> I am replying to a posting I saw on histonet regarding
> NBT/BCIP. I have used this chromogen a few times. At
> first I was told that I could not dehydrate with
> alcohol and claer with xylene if I used it. But since
> I was used to dehydrating and clearing using alcohol
> and xylene I went ahead and did it with NBT/BCP. I did
> not not see any fading of color. The color came out
> very well. I would also like to hear from others who
> have used it.
> James Mubiru
> UTHSCSA Texas
> 
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Agripina C. Suarez
Cardiovascular Research Laboratory
Room 292, McDonald Research Wing, St. Paul's Hospital
1081 Burrard St., Vancouver
B.C., Canada V6Z

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