Our research department has had some experience with HIF.
Antigen Retrieval: Citrate, 40 min. steam
Dilution: 1:1000, overnight
Secondary: an IgG2b for their specific clone of HIF
Tertiary: ABC

I have not worked with this antibody personally, but got this info from our
research department. I hope this helps you.

Patti Loykasek
Phenopath Laboratories
Seattle, WA