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<DIV>We have a Hacker linear stainer and have not had any problems with it. It is very long stainer but works quite well. We have the slide in each basket for 1 minute and 20 seconds. If you need the stain to be darker you can add another basket. Or if you need more deparaffinizing you can add another xylene. </DIV>
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<DIV>Thanks</DIV>
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<DIV>Jill</DIV>
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<DIV>Kaiser, CO </DIV>
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<DIV></DIV>>From: "Gladney, Diane C" <DIANE.GLADNEY@SE.AMEDD.ARMY.MIL>
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<DIV></DIV>>To: "'histonet@pathology.swmed.edu'" <HISTONET@PATHOLOGY.SWMED.EDU>
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<DIV></DIV>>Subject: H&E Auto Stainer
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<DIV></DIV>>Date: Thu, 12 Jul 2001 09:06:17 -0400
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<DIV></DIV>>Hi Fellow Histonetters,
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<DIV></DIV>>I have a Fisher Protocol Auto H&E Stainer w/ coverslipper that has been
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<DIV></DIV>>recalled by the manufacturer. I have had only very minor problems with this
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<DIV></DIV>>stainer and I hate to have to give it up. Fisher wants to replace the
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<DIV></DIV>>stainer with the Hacker MK II Linear Stainer. This stainer is almost 5 ft.
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<DIV></DIV>>long and each station cannot be individually timed. The time set is the time
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<DIV></DIV>>the slide rack will stay in each station. Reagent concentration determines
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<DIV></DIV>>the length of each step. Not only is the unit very large, but I can't seem
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<DIV></DIV>>to find anyone that currently has this stainer to evaluate the pros and
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<DIV></DIV>>cons. Since we cannot keep our Protocol stainer, I would like to have
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<DIV></DIV>>something that is about the same size and that I can control the time in
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<DIV></DIV>>each station. I know that Leica makes a stainer that would meet our needs
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<DIV></DIV>>but I urgently need some feedback on the Hacker stainer so that I and our
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<DIV></DIV>>pathologists can make a timely decision of whether to accept the Hacker
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<DIV></DIV>>stainer or opt for the money to replace the old stainer with another
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<DIV></DIV>>stainer. I know that there are some labs that are using this Hacker stainer.
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<DIV></DIV>>Is it really cost effective as the company claims? What about staining
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<DIV></DIV>>problems. I am the only Histotech in our lab and I have to do everything
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<DIV></DIV>>from accessioning, grossing, etc. You get the picture! My time is valuable
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<DIV></DIV>>and I can't spend unnecessary time each week making dilutions of stains and
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<DIV></DIV>>reagents just to do the H&E stain. Please give me some feedback as soon as
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<DIV></DIV>>possible.
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<DIV></DIV>>Thanks a million,
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<DIV></DIV>>Diane C. Gladney, HT(ASCP)
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<DIV></DIV>>Moncrief Army Community Hospital
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<DIV></DIV>>Dept. of Pathology, Histology Section
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<DIV></DIV>>Ft. Jackson, SC 29207-5600
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