I've noticed, on our cryostat (Leica CM3050), that sometimes you can get
alternate thick and thin sections if the angle of the blade and block are
such that the block can rub on the undersurface of the blade-holder, as it
passes after cutting a section. I don't know whether this might also be the
case on the cryostat that you use, in which case simply altering the angle
of cut may help.

Secondly make sure everything is done up tight, and the block is well
adhered to the chuck.

D. Choi
National Inst for Med Research, London UK
  ----- Original Message -----
  From: FANDORFP@aol.com
  To: Histonet@pathology.swmed.edu
  Sent: Wednesday, July 04, 2001 4:26 PM
  Subject: RE: Cryostat sectioning


  I have been having some touble while sectioning fresh frozen tissue this
  week. I have tried several different things to rememdy, nothing working.
Has
  anyone else experienced these problems? And how where they resolved.
  1. The tissue is splitting in half, all the way across the face of the
block.
  Not the up and down which would indicate to move the blade.
  2. Bad problem with thick and thin sections. I've sprayed the tissue to
bring
  down the temp and make it colder.
  3. Also when facing the block I have noticed, that there are ripples in
the
  block.
  4. Alot of Chatter, and the venetian blind.
  So anyone out there can help!!!!! I appreciate it!!! Thank -you ..