Re: Chrome Alum in Water Baths
|From:||Connie McManus <firstname.lastname@example.org>|
I realise you specified "routine"... but I do it both ways. Many times I
have had a recut request while cutting sections for IHC. I just put the
recut on a charged slide (Surgipath Snowcoat Xtra) and never have any
problems with them falling off. I use charged slides for all my brain
sections because they stay attached better than with gelatin & plain slides.
At 01:31 PM 6/28/01 -0700, Laurie Colbert wrote:
>How many people out there do not use anything (gelatin, chrome alum,
>etc.) in their waterbaths for routine surgicals? Do you use regular or
>positively-charged slides? How long do you leave slides on the hot
>plate and/or leave in the oven before staining? Do you have problems
>with the tissue falling off?
>Huntington Memorial Hospital
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