I realise you specified "routine"... but I do it both ways.  Many times I
have had a recut request while cutting sections for IHC.  I just put the
recut on a charged slide (Surgipath Snowcoat Xtra) and never have any
problems with them falling off.  I use charged slides for all my brain
sections because they stay attached better than with gelatin & plain slides.

Connie McManus


At 01:31 PM 6/28/01 -0700, Laurie Colbert wrote:
>How many people out there do not use anything (gelatin, chrome alum,
>etc.) in their waterbaths for routine surgicals?  Do you use regular or
>positively-charged slides?  How long do you leave slides on the hot
>plate and/or leave in the oven before staining?  Do you have problems
>with the tissue falling off?
>
>Laurie Colbert
>Huntington Memorial Hospital
>Pasadena, CA
>
>
>