Re: Chrome Alum in Water Baths

From:Laurence Reilly <>

G'day Laurie and Histonetters,
Our waterbaths contain only distilled water and we sub slides with
Glycerine/Albumen for brains, bones, hyperkeratotic skins etc.
The sections are dried in an oven with an internal fan at 60C for 30
minutes. We don't use hotplates.

           Regards, Laurie.

At 01:31  28/06/01 -0700, Laurie Colbert wrote:
>How many people out there do not use anything (gelatin, chrome alum,
>etc.) in their waterbaths for routine surgicals?  Do you use regular or
>positively-charged slides?  How long do you leave slides on the hot
>plate and/or leave in the oven before staining?  Do you have problems
>with the tissue falling off?
>Laurie Colbert
>Huntington Memorial Hospital
>Pasadena, CA
Mr.Laurie Reilly 
Histotechnologist.                         Ph 07 4781 4468
Physiology & Pharmacology                 Fax  07 4779 1526
Aust.Inst.of Tropical Vet.& Animal Sc.    
James Cook University           
       Townsville  Qld. 4811                            

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