Re: BrdU (and how and who to ask)
|From:||"J. A. Kiernan" <email@example.com>|
Your email was addressed to me alone, and I cannot
give you any useful suggestions.
For expert advice you should address your question to the
HistoNet listserver. Your email indicates that you subscribe
to that listserver. Probably someone looking at today's
Histonet questions will be able to help you. I do not know
the answer to your question, and I do not intend to go to
the library and occupy an hour looking it up.
Histonet is a useful resource for everyone in the field,
especially those who do not have a library or a shelf of
textbooks. Anyone can answer a question. All can read
the answer, and some will make comments that support,
supplement, modify, contradict etc. Questions and replies
are sent to everyone. Anyone can chip in with an answer
or a suggestion. You will learn more from this than
from picking out a regular answerer (me, about whom you
obviously know nothing) to ask a question that might
interest many other Histonetters.
I'm forwarding this reply to Histonet at large because
(a) it's of geenral interest, and
(b) someone reading it might effortlessly be able to
give you an answer to your question.
On Tue, 3 Jul 2001, Angeline Martin-Studdard wrote:
> Date: Tue, 03 Jul 2001 16:07:48 -0400
> Dear Dr. Kiernan,
> My name is Angeline Martin and I am employed at the Medical College of Georgia. I have had the pleasure of reading and utilizing your advice provided on the histonet many times. Our projects have involved the use of BrdU and I have been unable to obtain clarification on precisely how long this will be expressed in cells. We inject BrdU (IP) into young mice who have undergone some level of ischemia. The BrdU is easily detectable in the brain tissue sections. However, my concern is whether we are observing all new cell development from injection up until the time of sacrifice or is there only a "window" of opportunity for each cell in which the BrdU will be detectable?
> Any help you can provide will be greatly appreciated.
> Angeline M. Martin
> Department of Neurology
> Medical College of Georgia
> 1459 Laney-Walker Blvd.
> Augusta, GA 30912
> Ph: 706-721-6969
> Fax: 706-721-6839
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