Re: Brazilin

From:"Dr. Allen A. Smith" <asmith@mail.barry.edu>

Sodium iodide (NaI) is not an oxidizing agent.
Hematoxylin can be oxidized to hematein, and brazilin can be oxidized to
brasilein by sodium iodate (NaIO3).

Allen A. Smith, Ph.D.
School of Graduate Medical Sciences
   Podiatric Medicine and Surgery
Barry University
Miami Shores, Florida
----- Original Message -----
From: Paul Klosen <klosen@neurochem.u-strasbg.fr>
To: <histonet@pathology.swmed.edu>
Sent: Friday, July 06, 2001 5:12 AM
Subject: Brazilin


> Hi,
>
> A few weeks ago, I read about using brazilin instead of hematoxylin for a
> red nuclear stain and somebody (I apologize, I don't remember anymore who)
> suggested to prepare the stain like hematoxylin by replacing henatoxylin
> with Brazilin. I did this with our protocol for Mayers hemalun (1 g
> hematoxylin in 250 ml ssium alum, then adding 8 ml glacial acetic acid and
> 150 ml glycerol and water to 500 ml, oxidized with 0.1 g sodium iodide).
> Unfortunately the stain does not work.
>
> Can anyone post a full protocol for this Brazilin stain.
>
> Thanx in advance
>
> Paul
>                                                                          -
=-
>

                                                                       (o -)
O
> ===============================oOo==(_)==OOo=============================
> Paul Klosen, PhD
> CNRS UMR 7518 Neurobiologie des Fonctions Rythmiques et Saisonnieres
> Universite Louis Pasteur  12, rue de l'Universite
> F-67000 Strasbourg, FRANCE
> Tel. 03.90.24.05.01  Fax. 03.90.24.05.28
>
========================klosen@neurochem.u-strasbg.fr=======================
==
>
>





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