Re: B5

From:"J. A. Kiernan" <>

On Wed, 4 Jul 2001, Aidan Schurr wrote:

> I need to "borrow" someone's recipe for B5 fixative (as all my text books are *very* old and don't have it!).  Could someone help?

There can't be a "someone's" recipe for a named mixture.
According to Lillie & Fullmer (1976) p.52-53, B5 fixative is:

Water  90 ml
Mercuric chloride  6 g
Sodium acetate (anhydrous)  1.25 g
Formalin  10 ml.
(Filter if not clear. Fix for 12-24 hours.)

Does anyone know the original reference for "B5" ?
Why doesn't it have someone's name ?

John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London,  Canada   N6A 5C1

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