In our lab we stain for TRAP on decalcified FFPE tissues all the time. Yes,
it will not work on acid decaled materials but, will work beautifully on
EDTA decaled materials.

Jennifer Harvey  HT(ASCP) QIHC
University of Rochester

> ----------
> From: 	Patsy.Ruegg@UCHSC.edu
> Sent: 	Thursday, July 12, 2001 12:58 PM
> To: 	ret@gene.COM; histonet@pathology.swmed.edu
> Subject: 	RE: staining of mouse osteoclasts
> 
> There was an article in JOH about an antibody to acid phosphatase but I
> have
> not used it.  I still do enzyme histochemistry for osteoclasts (TRAP) but
> it
> does not work in decalcified ffpe tissue.  I use it on alcohol fixed,
> undecalcified, GMA embedded samples.
> Patsy Ruegg
> 
> 		-----Original Message-----
> 		From:	Robin E. Taylor [mailto:ret@gene.COM]
> 		Sent:	Thursday, July 12, 2001 8:14 AM
> 		To:	histonet@pathology.swmed.edu
> 		Cc:	ret@gene.COM
> 		Subject:	Re: staining of mouse osteoclasts
> 
> 		What about osteoblasts also?
> 
> 		Thanks.
> 
> 		Robin
> 
> 		Sarka Lhotak wrote:
> 
> 		> Hello Histonetters,
> 		>         would anybody have a technique for staining
> osteoclasts in mouse
> 		> bones, demineralized and paraffin embedded? A source of an
> antibody
> 		> (calcitonin receptor, RANK ??) would be appreciated, or
> any other
> 		> suggestions.
> 		>         Thanks a lot,
> 		>
> 		> Sarka Lhotak
> 		> Hamilton Regional Cancer Centre
>