RE: staining of mouse osteoclasts
From: | Patsy.Ruegg@UCHSC.edu |
There was an article in JOH about an antibody to acid phosphatase but I have
not used it. I still do enzyme histochemistry for osteoclasts (TRAP) but it
does not work in decalcified ffpe tissue. I use it on alcohol fixed,
undecalcified, GMA embedded samples.
Patsy Ruegg
-----Original Message-----
From: Robin E. Taylor [mailto:ret@gene.COM]
Sent: Thursday, July 12, 2001 8:14 AM
To: histonet@pathology.swmed.edu
Cc: ret@gene.COM
Subject: Re: staining of mouse osteoclasts
What about osteoblasts also?
Thanks.
Robin
Sarka Lhotak wrote:
> Hello Histonetters,
> would anybody have a technique for staining
osteoclasts in mouse
> bones, demineralized and paraffin embedded? A source of an
antibody
> (calcitonin receptor, RANK ??) would be appreciated, or
any other
> suggestions.
> Thanks a lot,
>
> Sarka Lhotak
> Hamilton Regional Cancer Centre
> McMa
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